Chapter title |
Radiation Damage in Macromolecular Crystallography
|
---|---|
Chapter number | 20 |
Book title |
Protein Crystallography
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7000-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6998-2, 978-1-4939-7000-1
|
Authors |
Elspeth F. Garman, Martin Weik |
Editors |
Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski |
Abstract |
Radiation damage inflicted on macromolecular crystals during X-ray diffraction experiments remains a limiting factor for structure solution, even when samples are cooled to cryotemperatures (~100 K). Efforts to establish mitigation strategies are ongoing and various approaches, summarized below, have been investigated over the last 15 years, resulting in a deeper understanding of the physical and chemical factors affecting damage rates. The recent advent of X-ray free electron lasers permits "diffraction-before-destruction" by providing highly brilliant and short (a few tens of fs) X-ray pulses. New fourth generation synchrotron sources now coming on line with higher X-ray flux densities than those available from third generation synchrotrons will bring the issue of radiation damage once more to the fore for structural biologists. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 40 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 23% |
Researcher | 9 | 23% |
Student > Bachelor | 4 | 10% |
Professor | 4 | 10% |
Student > Master | 2 | 5% |
Other | 3 | 8% |
Unknown | 9 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 20% |
Chemistry | 7 | 18% |
Agricultural and Biological Sciences | 6 | 15% |
Medicine and Dentistry | 4 | 10% |
Physics and Astronomy | 2 | 5% |
Other | 2 | 5% |
Unknown | 11 | 28% |