| Chapter title |
3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications
|
|---|---|
| Chapter number | 37 |
| Book title |
Decellularized Scaffolds and Organogenesis
|
| Published in |
Methods in molecular biology, May 2017
|
| DOI | 10.1007/7651_2017_37 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7655-3, 978-1-4939-7656-0
|
| Authors |
Rindone, Alexandra N., Nyberg, Ethan, Grayson, Warren L., Alexandra N. Rindone, Ethan Nyberg, Warren L. Grayson |
| Abstract |
Millions of patients worldwide require bone grafts for treatment of large, critically sized bone defects from conditions such as trauma, cancer, and congenital defects. Tissue engineered (TE) bone grafts have the potential to provide a more effective treatment than current bone grafts since they would restore fully functional bone tissue in large defects. Most bone TE approaches involve a combination of stem cells with porous, biodegradable scaffolds that provide mechanical support and degrade gradually as bone tissue is regenerated by stem cells. 3D-printing is a key technique in bone TE that can be used to fabricate functionalized scaffolds with patient-specific geometry. Using 3D-printing, composite polycaprolactone (PCL) and decellularized bone matrix (DCB) scaffolds can be produced to have the desired mechanical properties, geometry, and osteoinductivity needed for a TE bone graft. This book chapter will describe the protocols for fabricating and characterizing 3D-printed PCL:DCB scaffolds. Moreover, procedures for culturing adipose-derived stem cells (ASCs) in these scaffolds in vitro will be described to demonstrate the osteoinductivity of the scaffolds. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 78 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 22% |
| Student > Master | 14 | 18% |
| Student > Bachelor | 11 | 14% |
| Student > Doctoral Student | 5 | 6% |
| Researcher | 5 | 6% |
| Other | 8 | 10% |
| Unknown | 18 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 20 | 26% |
| Materials Science | 10 | 13% |
| Medicine and Dentistry | 8 | 10% |
| Biochemistry, Genetics and Molecular Biology | 7 | 9% |
| Agricultural and Biological Sciences | 3 | 4% |
| Other | 7 | 9% |
| Unknown | 23 | 29% |