Chapter title |
Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System
|
---|---|
Chapter number | 10 |
Book title |
Microbial Toxins
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6958-6_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6956-2, 978-1-4939-6958-6, 978-1-4939-6956-2, 978-1-4939-6958-6
|
Authors |
Janusz Boratyński, Bożena Szermer-Olearnik |
Editors |
Otto Holst |
Abstract |
Lipopolysaccharide (LPS, endotoxin, pyrogen) which is a component of the outer membrane of most Gram-negative bacteria is a troubling contaminant of crude bacteriophage suspension. Therefore, its removal is important for bacteriophage applications especially in preparations dedicated for use in therapy with bacterial infections treatment. The method presented here is used for extractive removal of endotoxins from bacteriophage preparations with a water immiscible solvent such as 1-octanol. During extraction most of the phage lytic activity is retained in the aqueous phase, while endotoxin accumulates in the organic solvent. The levels of endotoxin in the aqueous bacteriophage rich fraction are determine by Limulus Amebocyte Lysate or EndoLISA assay and are extremely low. |
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