| Chapter title |
Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering
|
|---|---|
| Chapter number | 31 |
| Book title |
Decellularized Scaffolds and Organogenesis
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/7651_2017_31 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7655-3, 978-1-4939-7656-0
|
| Authors |
Hodgson, Matthew J., Knutson, Christopher C., Momtahan, Nima, Cook, Alonzo D., Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, Alonzo D. Cook |
| Abstract |
Decellularization of whole porcine hearts followed by recellularization with fully differentiated cells made from patient-specific human induced pluripotent stem cells (iPSCs) may provide the ultimate solution for patients with heart failure. Decellularization is the process of completely disrupting all cells and removing the cellular components (e.g., antigenic proteins, lipids, DNA) from organic tissue, leaving only the extracellular matrix (ECM). The decellularization of porcine hearts can be accomplished in 24 h and results in 98% DNA removal with only 6 h of detergent exposure. Automatically controlling the pressure during decellularization reduces the detergent exposure time while still completely removing immunogenic cell debris. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 63 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 19% |
| Student > Master | 9 | 14% |
| Student > Bachelor | 7 | 11% |
| Researcher | 7 | 11% |
| Student > Doctoral Student | 3 | 5% |
| Other | 5 | 8% |
| Unknown | 20 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Agricultural and Biological Sciences | 6 | 10% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Environmental Science | 3 | 5% |
| Other | 10 | 16% |
| Unknown | 22 | 35% |