| Chapter title |
Mouse Skeletal Muscle Decellularization
|
|---|---|
| Chapter number | 28 |
| Book title |
Decellularized Scaffolds and Organogenesis
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/7651_2017_28 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7655-3, 978-1-4939-7656-0
|
| Authors |
Martina Piccoli, Caterina Trevisan, Edoardo Maghin, Chiara Franzin, Michela Pozzobon, Piccoli, Martina, Trevisan, Caterina, Maghin, Edoardo, Franzin, Chiara, Pozzobon, Michela |
| Abstract |
Natural acellular matrices obtained from decellularization procedures are biocompatible and non-immunogenic materials considered promising tools for regenerative medicine purposes. Before in vivo implantation, these matrices must be efficiently decellularized, removing all the cellular components to avoid any immunogenic reaction. At the same time, it is important to maintain the original three-dimensional structure of the specific tissue. Here we describe a method: (1) to decellularize mouse quadriceps using a detergent-enzymatic treatment (DET) and (2) to assess decellularization efficiency and scaffold properties. |
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Mendeley readers
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