| Chapter title |
Detection of mycoplasma contaminations.
|
|---|---|
| Chapter number | 1 |
| Book title |
Basic Cell Culture Protocols
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-128-8_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-127-1, 978-1-62703-128-8
|
| Authors |
Cord C Uphoff, Hans G Drexler, Uphoff CC, Drexler HG, Cord C. Uphoff, Hans G. Drexler, Uphoff, Cord C., Drexler, Hans G. |
| Abstract |
Mycoplasma contamination of cell lines is one of the major problems in cell culture technology. The -specific, sensitive, and reliable detection of mycoplasma contamination is an important part of mycoplasma control and should be an established method in every cell culture laboratory. New cell lines as well as cell lines in continuous culture must be tested in regular intervals. The polymerase chain reaction (PCR) methodology offers a fast and sensitive technique to monitor all cultures in a laboratory and can also be used to determine the contaminating species.The described assay can be performed in less than 3 hours, including sample preparation, DNA extraction, PCR run, and analysis of the PCR products. Special emphasis is given to steps taken to avoid false-negative results due to the presence of inhibitors of the Taq polymerase in the crude samples and the interpretation of the results. The technique can also be adapted to the requirements of the pharmacopoeia. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 75% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 50% |
| Members of the public | 1 | 25% |
| Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 3% |
| Switzerland | 1 | <1% |
| Malaysia | 1 | <1% |
| Brazil | 1 | <1% |
| France | 1 | <1% |
| Romania | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 119 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 39 | 30% |
| Student > Ph. D. Student | 24 | 19% |
| Student > Master | 15 | 12% |
| Student > Bachelor | 12 | 9% |
| Student > Postgraduate | 5 | 4% |
| Other | 11 | 9% |
| Unknown | 23 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 39 | 30% |
| Agricultural and Biological Sciences | 32 | 25% |
| Medicine and Dentistry | 12 | 9% |
| Immunology and Microbiology | 5 | 4% |
| Neuroscience | 5 | 4% |
| Other | 9 | 7% |
| Unknown | 27 | 21% |