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miRNomics: MicroRNA Biology and Computational Analysis

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Cover of 'miRNomics: MicroRNA Biology and Computational Analysis'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to MicroRNAs in Biological Systems
  3. Altmetric Badge
    Chapter 2 The Role of MicroRNAs in Biological Processes
  4. Altmetric Badge
    Chapter 3 The Role of MicroRNAs in Human Diseases
  5. Altmetric Badge
    Chapter 4 Introduction to bioinformatics.
  6. Altmetric Badge
    Chapter 5 MicroRNA and Noncoding RNA-Related Data Sources
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    Chapter 6 High-Throughput Approaches for MicroRNA Expression Analysis.
  8. Altmetric Badge
    Chapter 7 Introduction to Machine Learning
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    Chapter 8 Introduction to Statistical Methods for MicroRNA Analysis.
  10. Altmetric Badge
    Chapter 9 Computational and Bioinformatics Methods for MicroRNA Gene Prediction
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    Chapter 10 Machine Learning Methods for MicroRNA Gene Prediction
  12. Altmetric Badge
    Chapter 11 Functional, Structural, and Sequence Studies of MicroRNA
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    Chapter 12 Computational Methods for MicroRNA Target Prediction
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    Chapter 13 MicroRNA Target and Gene Validation in Viruses and Bacteria
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    Chapter 14 Gene Reporter Assay to Validate MicroRNA Targets in Drosophila S2 Cells.
  16. Altmetric Badge
    Chapter 15 Computational Prediction of MicroRNA Function and Activity
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    Chapter 16 Analysis of MicroRNA Expression Using Machine Learning.
  18. Altmetric Badge
    Chapter 17 MicroRNA Expression Landscapes in Stem Cells, Tissues, and Cancer
  19. Altmetric Badge
    Chapter 18 Master Regulators of Posttranscriptional Gene Expression Are Subject to Regulation
  20. Altmetric Badge
    Chapter 19 Use of MicroRNAs in Personalized Medicine
Attention for Chapter 14: Gene Reporter Assay to Validate MicroRNA Targets in Drosophila S2 Cells.
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Chapter title
Gene Reporter Assay to Validate MicroRNA Targets in Drosophila S2 Cells.
Chapter number 14
Book title
miRNomics: MicroRNA Biology and Computational Analysis
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-748-8_14
Pubmed ID
Book ISBNs
978-1-62703-747-1, 978-1-62703-748-8
Authors

Bünyamin Akgül, Cağdaş Göktaş, Çağdaş Göktaş, Akgül, Bünyamin, Göktaş, Çağdaş

Abstract

Bioinformatics programs have helped tremendously in identifying the targets of microRNAs, which are small noncoding RNAs that regulate gene expression posttranscriptionally. However, the partial complementarity between miRNAs and their targets hinders the accuracy of target prediction, necessitating the use of experimental validation procedures. Here, we describe a gene reporter assay typically used in our lab to validate putative miRNA-mRNA interactions in Drosophila S2 cells.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 11%
Unknown 8 89%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 22%
Student > Ph. D. Student 1 11%
Librarian 1 11%
Student > Doctoral Student 1 11%
Other 1 11%
Other 2 22%
Unknown 1 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 44%
Biochemistry, Genetics and Molecular Biology 2 22%
Computer Science 1 11%
Social Sciences 1 11%
Unknown 1 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 November 2013.
All research outputs
#19,015,492
of 23,577,654 outputs
Outputs from Methods in molecular biology
#8,199
of 13,410 outputs
Outputs of similar age
#233,464
of 309,121 outputs
Outputs of similar age from Methods in molecular biology
#302
of 576 outputs
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So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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