Chapter title |
Multiphoton/confocal Ca2+-imaging of inspiratory pre-Bötzinger complex neurons at the rostral or caudal surface of newborn rat brainstem slices.
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Chapter number | 17 |
Book title |
New Frontiers in Respiratory Control
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Published in |
Advances in experimental medicine and biology, January 2010
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DOI | 10.1007/978-1-4419-5692-7_17 |
Pubmed ID | |
Book ISBNs |
978-1-4419-5691-0, 978-1-4419-5692-7
|
Authors |
Nicoleta Bobocea, Araya Ruangkittisakul, Klaus Ballanyi |
Abstract |
Breathing is controled by neural networks of the pre-Bötzinger complex (preBötC). We have previously established that multiphoton/confocal Ca(2+) imaging is a potent tool for studying preBötC functions in transversal newborn rat brainstem slices. Here, we used such imaging to show that only 3 often dispersedly located preBötC neurons are typically inspiratory active per transversal imaging plane in slices with rostrally-exposed preBötC contrary to rhythmic optical activity in 11 densely aggregated neurons in slices with the preBötC at the caudal margin. In both slice types, glutamate raises Ca(2+) in >30 cells (both neurons and glia). Factors are discussed that may be involved in the spatial inhomogeneity of superficially located active inspiratory preBötC neurons in both slice types. |
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