Chapter title |
QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation Into Liposomes
|
---|---|
Chapter number | 5 |
Book title |
Vaccine Adjuvants
|
Published in |
Methods in molecular biology, October 2016
|
DOI | 10.1007/978-1-4939-6445-1_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6443-7, 978-1-4939-6445-1
|
Authors |
Livia Brunner, Christophe Barnier-Quer, Nicolas Collin |
Editors |
Christopher B. Fox |
Abstract |
QS-21, a saponin extracted from the tree Quillaja saponaria Molina, is a vaccine adjuvant which has been shown to elicit robust antibody and cell-mediated immune responses in a variety of preclinical and clinical studies [1]. Its purification from the natural source is a lengthy and difficult process. The commercially available saponin mixture Quil-A(®) is a fraction of the bark extract containing a variety of saponins, including QS-21. In order to facilitate access to QS-21 at laboratory-scale amounts, we propose here a method of purification of QS-21 starting from Quil-A(®). In addition, we describe a protocol to appropriately formulate QS-21 into cholesterol-containing, neutral liposomes which are known to decrease QS-21's hemolytic activity while retaining the adjuvant effect. Methods for the physicochemical characterization of purified QS-21 and of the QS-21/liposome formulations are also described. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 24% |
Researcher | 3 | 18% |
Professor | 2 | 12% |
Student > Ph. D. Student | 1 | 6% |
Unknown | 7 | 41% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 3 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 12% |
Chemical Engineering | 1 | 6% |
Chemistry | 1 | 6% |
Engineering | 1 | 6% |
Other | 0 | 0% |
Unknown | 9 | 53% |