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Structural Proteomics

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Cover of 'Structural Proteomics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Target selection for structural genomics: an overview.
  3. Altmetric Badge
    Chapter 2 Structural Proteomics
  4. Altmetric Badge
    Chapter 3 Target selection: triage in the structural genomics battlefield.
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    Chapter 4 Data management in structural genomics: an overview.
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    Chapter 5 Data deposition and annotation at the worldwide protein data bank.
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    Chapter 6 Prediction of protein disorder.
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    Chapter 7 Structural Proteomics
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    Chapter 8 Protein structure modeling with MODELLER.
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    Chapter 9 High throughput cloning with restriction enzymes.
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    Chapter 10 Automated recombinant protein expression screening in Escherichia coli.
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    Chapter 11 From no expression to high-level soluble expression in Escherichia coli by screening a library of the target proteins with randomized N-termini.
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    Chapter 12 Application of high-throughput methodologies to the expression of recombinant proteins in E. coli.
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    Chapter 13 A high throughput platform for eukaryotic genes.
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    Chapter 14 High throughput production of recombinant human proteins for crystallography.
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    Chapter 15 Assembly of protein complexes by coexpression in prokaryotic and eukaryotic hosts: an overview.
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    Chapter 16 Cell-free protein synthesis for analysis by NMR spectroscopy.
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    Chapter 17 A medium or high throughput protein refolding assay.
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    Chapter 18 Structural proteomics of membrane proteins: a survey of published techniques and design of a rational high throughput strategy.
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    Chapter 19 Structural Proteomics
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    Chapter 20 High throughput methods for analyzing transition metals in proteins on a microgram scale.
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    Chapter 21 High throughput screening of purified proteins for enzymatic activity.
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    Chapter 22 Strategies for improving crystallization success rates.
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    Chapter 23 Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomics.
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    Chapter 24 Fluorescence approaches to growing macromolecule crystals.
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    Chapter 25 Efficient macromolecular crystallization using microfluidics and randomized design of screening reagents.
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    Chapter 26 Increasing protein crystallization screening success with heterogeneous nucleating agents.
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    Chapter 27 High throughput pH optimization of protein crystallization.
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    Chapter 28 Automated structure solution with the PHENIX suite.
  30. Altmetric Badge
    Chapter 29 NMR screening for rapid protein characterization in structural proteomics.
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    Chapter 30 Microcoil NMR spectroscopy: a novel tool for biological high throughput NMR spectroscopy.
  32. Altmetric Badge
    Chapter 31 Structural Proteomics
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    Chapter 32 Structural Proteomics
  34. Altmetric Badge
    Chapter 33 Structural genomics of pathogenic protozoa: an overview.
  35. Altmetric Badge
    Chapter 34 High throughput crystallography at SGC Toronto: an overview.
  36. Altmetric Badge
    Chapter 35 The structural biology and genomics platform in strasbourg: an overview.
  37. Altmetric Badge
    Chapter 36 Bacterial structural genomics initiative: overview of methods and technologies applied to the process of structure determination.
  38. Altmetric Badge
    Chapter 37 High throughput protein production and crystallization at NYSGXRC.
  39. Altmetric Badge
    Chapter 38 Overview of the pipeline for structural and functional characterization of macrophage proteins at the university of queensland.
  40. Altmetric Badge
    Chapter 39 Structural Genomics of the Bacterial Mobile Metagenome: an Overview
Attention for Chapter 34: High throughput crystallography at SGC Toronto: an overview.
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Chapter title
High throughput crystallography at SGC Toronto: an overview.
Chapter number 34
Book title
Structural Proteomics
Published in
Methods in molecular biology, December 2007
DOI 10.1007/978-1-60327-058-8_34
Pubmed ID
Book ISBNs
978-1-58829-809-6, 978-1-60327-058-8
Authors

Bochkarev A, Tempel W, Alexey Bochkarev, Wolfram Tempel

Editors

Bostjan Kobe, Mitchell Guss, Thomas Huber

Abstract

The completion of the human genome allows the analysis, for the first time, of biological systems in the context of entire gene families. For enzymes, this approach permits the exploration of complex substrate specificity networks that often exhibit considerable overlap within and between protein families. The case for a family-based approach to protein studies is compelling, given the prospect of exploiting these specificities for various purposes, such as the development of therapeutic reagents. The Structural Genomics Consortium (SGC) was created to determine the structures of proteins with relevance to human health and place the structures into the public domain without restriction on use. The SGC operates out of the Universities of Toronto and Oxford, and Karolinska Institutet, each working on nonoverlapping protein target lists. The SGC focus on human protein families requires a repertoire of crystallography methods that differ from those adopted by structural genomics projects that are focused on filling out protein fold space. The key differences are heavier reliance on in house x-ray sources for diffraction data collection and predominant use of molecular replacement for phase determination. As projects such as the US Protein Structure Initiative and others fill the PDB with representatives of most major fold families, the SGC approach will become an increasingly useful model for many structural biology laboratories in the future. Technical details of the flow of samples and data within the high throughput (HTP) environment at SGC Toronto are presented, and provide a useful paradigm for the organization of collaborative or shared x-ray instrumentation facilities.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 11%
Unknown 8 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 33%
Researcher 3 33%
Professor 2 22%
Student > Master 1 11%
Student > Bachelor 1 11%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 78%
Biochemistry, Genetics and Molecular Biology 2 22%
Chemistry 1 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 November 2014.
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#20,243,777
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Outputs from Methods in molecular biology
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#150,924
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Outputs of similar age from Methods in molecular biology
#82
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