Chapter title |
Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells.
|
---|---|
Chapter number | 25 |
Book title |
Fluorescence Spectroscopy and Microscopy
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-62703-649-8_25 |
Pubmed ID | |
Book ISBNs |
978-1-62703-648-1, 978-1-62703-649-8
|
Authors |
Xiaoxiao Ma, Yong Hwee Foo, Thorsten Wohland, Ma, Xiaoxiao, Foo, Yong Hwee, Wohland, Thorsten |
Abstract |
Fluorescence cross-correlation spectroscopy (FCCS) is a single-molecule sensitive technique to quantitatively study interactions among fluorescently tagged biomolecules. Besides the initial implementation as dual-color FCCS (DC-FCCS), FCCS has several powerful derivatives, including single-wavelength FCCS (SW-FCCS), two-photon FCCS (TP-FCCS), and pulsed interleaved excitation FCCS (PIE-FCCS). However, to apply FCCS successfully, one needs to be familiar with procedures ranging from fluorescent labeling, instrumentation setup and alignment, sample preparation, and data analysis. Here, we describe the procedures to apply FCCS in various biological samples ranging from live cells to in vivo measurements, with the focus on DC-FCCS and SW-FCCS. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Hungary | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Portugal | 1 | 2% |
France | 1 | 2% |
Unknown | 45 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 26% |
Researcher | 10 | 21% |
Student > Master | 5 | 11% |
Student > Bachelor | 3 | 6% |
Student > Doctoral Student | 2 | 4% |
Other | 6 | 13% |
Unknown | 9 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 23% |
Biochemistry, Genetics and Molecular Biology | 9 | 19% |
Chemistry | 6 | 13% |
Physics and Astronomy | 6 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 3 | 6% |
Unknown | 11 | 23% |