Chapter title |
Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture
|
---|---|
Chapter number | 2 |
Book title |
Organ Regeneration
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6949-4_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6947-0, 978-1-4939-6949-4
|
Authors |
Kuwahara, Atsushi, Nakano, Tokushige, Eiraku, Mototsugu, Atsushi Kuwahara, Tokushige Nakano, Mototsugu Eiraku |
Editors |
Takashi Tsuji |
Abstract |
A three-dimensional (3D) tissue generated in vitro is a promising source to study developmental biology and regenerative medicine. In the last decade, Yoshiki Sasai's group have developed a 3D stem cell culture technique known as SFEBq and demonstrated that embryonic stem cells (ESCs) have an ability to self-organize stratified neural tissue including 3D-retina. Furthermore, we have reported that ESC-derived retinal tissue can form an optic cup and a ciliary margin, which are unique structures in the developing retina. In this review, we focus on self-organizing culture technique to generate 3D-retina from human ESCs. |
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Unknown | 2 | 14% |