Chapter title |
Directed Differentiation of Mouse Embryonic Stem Cells Into Inner Ear Sensory Epithelia in 3D Culture
|
---|---|
Chapter number | 6 |
Book title |
Organ Regeneration
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6949-4_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6947-0, 978-1-4939-6949-4
|
Authors |
Nie, Jing, Koehler, Karl R., Hashino, Eri, Jing Nie, Karl R. Koehler, Eri Hashino |
Editors |
Takashi Tsuji |
Abstract |
The inner ear sensory epithelium harbors mechanosensory hair cells responsible for detecting sound and maintaining balance. This protocol describes a three-dimensional (3D) culture system that efficiently generates inner ear sensory epithelia from aggregates of mouse embryonic stem (mES) cells. By mimicking the activations and repressions of key signaling pathways during in vivo inner ear development, mES cell aggregates are sequentially treated with recombinant proteins and small molecule inhibitors for activating or inhibiting the Bmp, TGFβ, Fgf, and Wnt signaling pathways. These stepwise treatments promote mES cells to sequentially differentiate into epithelia representing the non-neural ectoderm, preplacodal ectoderm, otic placodal ectoderm, and ultimately, the hair cell-containing sensory epithelia. The derived hair cells are surrounded by a layer of supporting cells and are innervated by sensory neurons. This in vitro inner ear organoid culture system may serve as a valuable tool in developmental and physiological research, disease modeling, drug testing, and potential cell-based therapies. |
X Demographics
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Unknown | 2 | 100% |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 28 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 4 | 14% |
Professor | 4 | 14% |
Researcher | 4 | 14% |
Student > Master | 4 | 14% |
Student > Bachelor | 2 | 7% |
Other | 4 | 14% |
Unknown | 6 | 21% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 9 | 32% |
Agricultural and Biological Sciences | 5 | 18% |
Neuroscience | 2 | 7% |
Medicine and Dentistry | 2 | 7% |
Unspecified | 1 | 4% |
Other | 3 | 11% |
Unknown | 6 | 21% |