Chapter title |
Liver Regeneration Using Cultured Liver Bud
|
---|---|
Chapter number | 15 |
Book title |
Organ Regeneration
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6949-4_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6947-0, 978-1-4939-6949-4
|
Authors |
Sekine, Keisuke, Takebe, Takanori, Taniguchi, Hideki, Keisuke Sekine, Takanori Takebe, Hideki Taniguchi |
Editors |
Takashi Tsuji |
Abstract |
Here, we describe a protocol to develop a three-dimensional (3D) liver bud-like tissue from human iPSCs in vitro. This method mainly consists of two parts: (1) hepatic endoderm (HE) differentiation from human iPSCs in 2D culture and (2) co-culturing iPSC-HE with endothelial and mesenchymal cells. First, iPSCs were differentiated into definitive endoderm (DE) cells, and the DE cells were differentiated into HE cells, which were then co-cultured with endothelial cells and mesenchymal cells on Matrigel-coated plastic plates or micropattern plates. The cells rapidly condensed to generate 3D tissue masses. We named these iPSC liver buds (iPSC-LBs) because they resemble the developing liver bud from the perspective of gene expression, cell proliferation, and cell proportion. This liver bud culture system provides a novel approach for future clinical applications, for drug development, and as a tool for studying human development. |
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Unknown | 2 | 22% |