Chapter title |
Germline stem cells and their regulation in the nematode Caenorhabditis elegans.
|
---|---|
Chapter number | 3 |
Book title |
Transcriptional and Translational Regulation of Stem Cells
|
Published in |
Advances in experimental medicine and biology, January 2013
|
DOI | 10.1007/978-94-007-6621-1_3 |
Pubmed ID | |
Book ISBNs |
978-9-40-076620-4, 978-9-40-076621-1
|
Authors |
Aaron Kershner, Sarah L Crittenden, Kyle Friend, Erika B Sorensen, Douglas F Porter, Judith Kimble, Sarah L. Crittenden, Erika B. Sorensen, Douglas F. Porter |
Abstract |
C. elegans germline stem cells exist within a stem cell pool that is maintained by a single-celled mesenchymal niche and Notch signaling. Downstream of Notch signaling, a regulatory network governs stem cells and differentiation. Central to that network is the FBF RNA-binding protein, a member of the widely conserved PUF family that functions by either of two broadly conserved mechanisms to repress its target mRNAs. Without FBF, germline stem cells do not proliferate and they do not maintain their naïve, undifferentiated state. Therefore, FBF is a pivotal regulator of germline self-renewal. Validated FBF targets include several key differentiation regulators as well as a major cell cycle regulator. A genomic analysis identifies many other developmental and cell cycle regulators as likely FBF targets and suggests that FBF is a broad-spectrum regulator of the genome with >1,000 targets. A comparison of the FBF target list with similar lists for human PUF proteins, PUM1 and PUM2, reveals ∼200 shared targets. The FBF hub works within a network controlling self-renewal vs. differentiation. This network consists of classical developmental cell fate regulators and classical cell cycle regulators. Recent results have begun to integrate developmental and cell cycle regulation within the network. The molecular dynamics of the network remain a challenge for the future, but models are proposed. We suggest that molecular controls of C. elegans germline stem cells provide an important model for controls of stem cells more broadly. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 30% |
Student > Ph. D. Student | 2 | 20% |
Other | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Unknown | 3 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 40% |
Agricultural and Biological Sciences | 2 | 20% |
Environmental Science | 1 | 10% |
Unknown | 3 | 30% |