Chapter title |
Studying the T Cell-Astrocyte Immune Synapse
|
---|---|
Chapter number | 32 |
Book title |
The Immune Synapse
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6881-7_32 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6879-4, 978-1-4939-6881-7
|
Authors |
George P. Cribaro, Elena Saavedra-López, Paola V. Casanova, Laura Rodríguez, Carlos Barcia |
Editors |
Cosima T. Baldari, Michael L. Dustin |
Abstract |
In this chapter, we describe the technical details to visualize and analyze effector immunological synapses between T cells and astrocytes in the brain with high-resolution confocal imaging. This procedure is critical for the optimal and even penetration of labeling antibodies within the nerve tissue to obtain accurate staining and allow a uniform three-dimensional analysis of the T cell-astrocyte interactions. We emphasize here the comprehensive exploration of the tissue and analysis with confocal microscope as well as the display of microanatomical details of the three-dimensional reconstruction for interface visualization (including peripheral and central supramolecular activation clusters, effector molecules, and other organelles such as microtubule organizing centers (MTOCs) and Golgi apparatus). |
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 2 | 25% |
Librarian | 1 | 13% |
Student > Bachelor | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Student > Master | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |
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Unknown | 2 | 25% |