Chapter title |
Modulation of the host skeletal muscle niche for donor satellite cell grafting.
|
---|---|
Chapter number | 15 |
Book title |
Stem Cell Niche
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-508-8_15 |
Pubmed ID | |
Book ISBNs |
978-1-62703-507-1, 978-1-62703-508-8
|
Authors |
Luisa Boldrin, Jennifer E. Morgan |
Abstract |
Skeletal muscle tissue has a remarkable capability of regenerating in pathological conditions or after injury. The principal muscle stem cells, satellite cells, are responsible for this prompt and efficient process. Normally quiescent in their niches underneath the basal lamina of each muscle fiber, satellite cells become activated to repair or form new fibers. Ideally, healthy donor stem cells could be transplanted to regenerate the skeletal muscle tissue to repair a genetic defect. However, to be efficient, cell grafting requires modulation of the host muscle environment to allow homing of, and regeneration by, donor satellite cells. Here, we provide methods to modulate the host mouse muscle environment in order to destroy or preserve the muscle niche before transplanting donor satellite cells. We also describe methods to investigate donor-derived muscle regeneration and self-renewal. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 33% |
United States | 1 | 33% |
Norway | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 38% |
Student > Bachelor | 4 | 25% |
Professor | 1 | 6% |
Student > Master | 1 | 6% |
Student > Ph. D. Student | 1 | 6% |
Other | 2 | 13% |
Unknown | 1 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 38% |
Biochemistry, Genetics and Molecular Biology | 5 | 31% |
Medicine and Dentistry | 2 | 13% |
Unknown | 3 | 19% |