| Chapter title |
Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies
|
|---|---|
| Chapter number | 15 |
| Book title |
Synthetic Messenger RNA and Cell Metabolism Modulation
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-260-5_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-259-9, 978-1-62703-260-5
|
| Authors |
Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Ãzlem Türeci, Ugur Sahin, Özlem Türeci, Diken, Mustafa, Kreiter, Sebastian, Selmi, Abderraouf, Türeci, Özlem, Sahin, Ugur |
| Abstract |
Synthetic antigen-encoding mRNA is increasingly exploited as a tool for delivery of genetic information of complete antigens into professional antigen presenting dendritic cells for HLA haplotype-independent antigen-specific vaccination against cancer. Two strategies for mRNA-based antitumor vaccination have emerged into the clinical setting. One is transfection of autologous dendritic cells with synthetic mRNA for adoptive transfer into the patient. The other is direct injection of naked synthetic mRNA. Both methods have proven to be feasible and safe and to elicit antigen-specific immune responses. The design of novel synthetic vaccines employing synthetic mRNA requires further in-depth investigation of its bioavailability and immune pharmacology. This chapter summarizes the state-of-art in this field and describes methods elementary for preclinical studies of mRNA-based antitumor vaccine protocols. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 14% |
| Professor | 3 | 10% |
| Other | 3 | 10% |
| Student > Ph. D. Student | 3 | 10% |
| Student > Master | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 12 | 41% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 5 | 17% |
| Agricultural and Biological Sciences | 4 | 14% |
| Biochemistry, Genetics and Molecular Biology | 3 | 10% |
| Medicine and Dentistry | 3 | 10% |
| Philosophy | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 13 | 45% |