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PCR Detection of Microbial Pathogens

Overview of attention for book
Cover of 'PCR Detection of Microbial Pathogens'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Real-Time Quantitative PCR, Pathogen Detection and MIQE.
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    Chapter 2 Overcoming Inhibition in Real-Time Diagnostic PCR
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    Chapter 3 Quality in the Molecular Microbiology Laboratory
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    Chapter 4 Pre-analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood
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    Chapter 5 Detection of Bacterial Contamination in Platelet Concentrates Using Flow Cytometry and Real-Time PCR Methods
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    Chapter 6 Multiplex Real-Time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton–Valentine Leukocidin from Clinical Samples
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    Chapter 7 PCR Detection of Haemophilus influenzae from Respiratory Specimens
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    Chapter 8 Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-Time PCR
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    Chapter 9 PCR Detection of Microbial Pathogens
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    Chapter 10 PCR Detection of Microbial Pathogens
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    Chapter 11 Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia
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    Chapter 12 Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens
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    Chapter 13 Direct Detection of Mycobacterium ulcerans in Clinical Specimens and Environmental Samples
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    Chapter 14 PCR Detection of Microbial Pathogens
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    Chapter 15 Simultaneous Direct Identification of Genital Microorganisms in Voided Urine Using Multiplex PCR-Based Reverse Line Blot Assays.
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    Chapter 16 Diagnosis of Clostridium difficile Infection Using Real-Time PCR
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    Chapter 17 PCR Detection of Microbial Pathogens
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    Chapter 18 Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli Using Loop-Mediated Isothermal Amplification
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    Chapter 19 PCR Detection of Helicobacter pylori in Clinical Samples.
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    Chapter 20 Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-Time PCR
  22. Altmetric Badge
    Chapter 21 Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic
Attention for Chapter 1: Real-Time Quantitative PCR, Pathogen Detection and MIQE.
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Chapter title
Real-Time Quantitative PCR, Pathogen Detection and MIQE.
Chapter number 1
Book title
PCR Detection of Microbial Pathogens
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-60327-353-4_1
Pubmed ID
Book ISBNs
978-1-60327-352-7, 978-1-60327-353-4
Authors

Gemma Johnson, Tania Nolan, Stephen A. Bustin

Editors

Mark Wilks

Abstract

Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design and validation as well as appropriate data analysis and the "Minimal Information for the Publication of real-time PCR" (MIQE) guidelines aim to improve both the reliability of assay design as well as the transparency of reporting, essential conditions if qPCR is to remain the benchmark technology for molecular diagnosis.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 144 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
Mexico 1 <1%
Unknown 141 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 24 17%
Student > Ph. D. Student 23 16%
Researcher 22 15%
Student > Master 19 13%
Other 9 6%
Other 15 10%
Unknown 32 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 33 23%
Agricultural and Biological Sciences 30 21%
Immunology and Microbiology 11 8%
Medicine and Dentistry 10 7%
Environmental Science 6 4%
Other 15 10%
Unknown 39 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 January 2014.
All research outputs
#15,274,524
of 22,714,025 outputs
Outputs from Methods in molecular biology
#5,303
of 13,079 outputs
Outputs of similar age
#181,528
of 280,752 outputs
Outputs of similar age from Methods in molecular biology
#174
of 341 outputs
Altmetric has tracked 22,714,025 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,079 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,752 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 341 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.