Transmembrane β-Barrel Proteins
Springer US
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| Chapter title |
Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel.
|
|---|---|
| Book title |
Transmembrane β-Barrel Proteins
|
| Published in |
Methods in molecular biology, January 2024
|
| DOI | 10.1007/978-1-0716-3734-0_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-07-163733-3, 978-1-07-163734-0
|
| Authors |
Conners, Rebecca, McLaren, Mathew, Russel, Marjorie, Gold, Vicki A M, Gold, Vicki A. M. |
| Abstract |
Secretin proteins form pores in the outer membranes of Gram-negative bacteria, and as such provide a means of transporting a wide variety of molecules out of or in to the cell. They are important components of several different bacterial secretion systems, surface filament assembly machineries, and virus assembly complexes. Despite accommodating a diverse assortment of molecules, including virulence factors, folded proteins, and whole viruses, the secretin family of proteins is highly conserved, particularly in their membrane-embedded β-barrel domain. We describe here a protocol for the expression, purification and cryo-EM structural determination of the pIV secretin from the Ff family of filamentous bacteriophages. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 1 | 50% |
| Unspecified | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2024.
All research outputs
#17,364,523
of 25,482,409 outputs
Outputs from Methods in molecular biology
#6,035
of 14,220 outputs
Outputs of similar age
#179,699
of 339,961 outputs
Outputs of similar age from Methods in molecular biology
#114
of 275 outputs
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