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Metabolomics: From Fundamentals to Clinical Applications

Overview of attention for book
Attention for Chapter 3: Nuclear Magnetic Resonance Strategies for Metabolic Analysis
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Chapter title
Nuclear Magnetic Resonance Strategies for Metabolic Analysis
Chapter number 3
Book title
Metabolomics: From Fundamentals to Clinical Applications
Published in
Advances in experimental medicine and biology, January 2017
DOI 10.1007/978-3-319-47656-8_3
Pubmed ID
Book ISBNs
978-3-31-947655-1, 978-3-31-947656-8
Authors

Clement Heude, Jay Nath, John Bosco Carrigan, Christian Ludwig

Editors

Alessandra Sussulini

Abstract

NMR spectroscopy is a powerful tool for metabolomic studies, offering highly reproducible and quantitative analyses. This burgeoning field of NMR metabolomics has been greatly aided by the development of modern spectrometers and software, allowing high-throughput analysis with near real-time feedback. Whilst one-dimensional proton (1D-(1)H) NMR analysis is best described and remains most widely used, a plethora of alternative NMR techniques are now available that offer additional chemical and structural information and resolve many of the limitations of conventional 1D-(1)H NMR such as spectral overlay. In this book chapter, we review the principal concepts of practical NMR spectroscopy, from common sample preparation protocols to the benefits and theoretical concepts underpinning the commonly used pulse sequences. Finally, as a case study to highlight the utility of NMR as a method for metabolomic investigation, we have detailed how NMR has been used to gain valuable insight into the metabolism occurring in kidneys prior to transplantation and the potential implications of this.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 28%
Student > Bachelor 4 16%
Student > Master 4 16%
Other 2 8%
Researcher 2 8%
Other 4 16%
Unknown 2 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 24%
Agricultural and Biological Sciences 4 16%
Medicine and Dentistry 4 16%
Chemistry 3 12%
Pharmacology, Toxicology and Pharmaceutical Science 2 8%
Other 4 16%
Unknown 2 8%