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The Plant Endoplasmic Reticulum

Overview of attention for book
The Plant Endoplasmic Reticulum
Springer US

Table of Contents

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    Book Overview
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    Chapter 1 Make It Shine: Labelling the ER for Light and Fluorescence Microscopy.
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    Chapter 2 Electron Microscopy Techniques for 3D Plant ER Imaging.
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    Chapter 3 Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy.
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    Chapter 4 Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques.
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    Chapter 5 Quantitation of ER Morphology and Dynamics.
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    Chapter 6 Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination.
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    Chapter 7 Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules.
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    Chapter 8 Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis.
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    Chapter 9 Preparation of ER Microsomes from Arabidopsis thaliana.
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    Chapter 10 ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis.
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    Chapter 11 2 in 1 Vectors Improve in Planta BiFC and FRET Analysis.
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    Chapter 12 Immunoprecipitation and FRET-FLIM to Determine Metabolons on the Plant ER.
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    Chapter 13 Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells.
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    Chapter 14 Protein Biosynthesis and Maturation in the ER.
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    Chapter 15 ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS).
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    Chapter 16 Analysis of Protein Glycosylation in the ER.
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    Chapter 17 Unfolded Protein Response in Arabidopsis.
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    Chapter 18 Imaging the ER and Endomembrane System in Cereal Endosperm.
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    Chapter 19 Multi-omics Resources for Understanding Gene Regulation in Response to ER Stress in Plants.
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    Chapter 20 Variable-Angle Epifluorescence Microscopy for Single-Particle Tracking in the Plant ER.
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    Chapter 21 Use of Super-Resolution Live Cell Imaging to Distinguish Endoplasmic Reticulum: Nuclear Envelope Subcellular Localization.
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    Chapter 22 Using ER-Targeted Photoconvertible Fluorescent Proteins in Living Plant Cells.
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    Chapter 23 In Vivo Analysis of ER-Associated Protein Degradation and Ubiquitination in Arabidopsis thaliana.
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    Chapter 24 Overexpression of Endoplasmic Reticulum Proteins from Arabidopsis thaliana in Baculovirus.
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    Chapter 25 Observing ER Dynamics over Long Timescales Using Light Sheet Fluorescence Microscopy.
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    Chapter 26 Gene Stacking and Stoichiometric Expression of ER-Targeted Constructs Using "2A" Self-Cleaving Peptides.
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    Chapter 27 Automated Segmentation and 3D Reconstruction of Different Membranes from Confocal Z-Stacks.
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    Chapter 28 Visualizing Orientation and Topology of ER Membrane Proteins In Planta.
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    Chapter 29 Organ-Specific Microsomes from Dark-Grown Hypocotyls of Arabidopsis thaliana.
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    Chapter 30 Studying Secretory Protein Synthesis in Nicotiana benthamiana Protoplasts.
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    Chapter 31 Analyzing Photoactivation with Diffusion Models to Study Transport in the Endoplasmic Reticulum Network.
Attention for Chapter 4: Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques.
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Chapter title
Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques.
Chapter number 4
Book title
The Plant Endoplasmic Reticulum
Published by
Humana, New York, NY, January 2024
DOI 10.1007/978-1-0716-3710-4_4
Pubmed ID
38411805
Book ISBNs
978-1-07-163709-8, 978-1-07-163710-4
Authors

Karen Bell, Karl Oparka, Kirsten Knox

Abstract

The plant endoplasmic reticulum (ER) forms several specialized structures. These include the sieve element reticulum (SER) and the desmotubule formed as the ER passes through plasmodesmata. Imaging both of these structures has been inhibited by the resolution limits of light microscopy and their relatively inaccessible locations, combined with the fragile nature of the ER. Here we describe methods to view desmotubules in live cells under 3D-structured illumination microscopy (3D-SIM) and methods to fix and prepare phloem tissue for both 3D-SIM and transmission electron microscopy (TEM), which preserve the fragile structure and allow the detailed imaging of the SER.

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