| Chapter title |
Molecular Genetic Tests for FLT3, NPM1, and CEBPA in Acute Myeloid Leukemia.
|
|---|---|
| Chapter number | 7 |
| Book title |
Hematological Malignancies
|
| Published in |
Methods in molecular biology, May 2013
|
| DOI | 10.1007/978-1-62703-357-2_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-356-5, 978-1-62703-357-2
|
| Authors |
Zhang Q, Bai S, Vance GH, Qing Zhang, Shaochun Bai, Gail H. Vance, Zhang, Qing, Bai, Shaochun, Vance, Gail H. |
| Abstract |
Patients with acute myeloid leukemia (AML) and a normal karyotype constitute the single largest cytogenetic group of AML. It is important to identify prognostic markers that predict patients' outcome more precisely. The presence of mutations in FLT3 (FMS-like tyrosine kinase 3), NPM1 (Nucleophosmin), and CEBPA (CCAAT/enhancer-binding protein alpha) genes hold prognostic significance in patients with AML and normal cytogenetics. Therefore, mutation identification may help to optimize therapeutic approaches in this group of patients. Polymerase chain reaction (PCR)-based fragment length analysis for mutations in FLT3 and NPM1 has been shown to be a fast and sensitive method, while nucleotide sequencing represents a gold standard for CEBPA heterogeneous mutational screening. We describe both fragment length assay and sequencing methods for mutational analysis of these three genes. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 15% |
| Student > Postgraduate | 2 | 15% |
| Student > Doctoral Student | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Other | 2 | 15% |
| Unknown | 4 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 23% |
| Agricultural and Biological Sciences | 2 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Computer Science | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 4 | 31% |