Chapter title |
Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells.
|
---|---|
Chapter number | 1 |
Book title |
Recombinant Glycoproteins
|
Published in |
Methods in molecular biology, January 2024
|
DOI | 10.1007/978-1-0716-3666-4_1 |
Pubmed ID | |
Book ISBNs |
978-1-07-163665-7, 978-1-07-163666-4
|
Authors |
Meola, Annalisa, Guardado-Calvo, Pablo |
Abstract |
Hantaviruses, are rodent-borne viruses found worldwide that are transmitted to humans through inhalation of contaminated excreta. They can cause a renal or a pulmonary syndrome, depending on the virus, and no effective treatment is currently available for either of these diseases. Hantaviral particles are covered by a protein lattice composed of two glycoproteins (Gn and Gc) that mediate adsorption to target cells and fusion with endosomal membranes, making them prime targets for neutralizing antibodies. Here we present the methodology to produce soluble recombinant glycoproteins in different conformations, either alone or as a stabilized Gn/Gc complex, using stably transfected Drosophila S2 cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |