Chapter title |
Gene Expression Analysis of Shoot Apical Meristem Cell Types
|
---|---|
Chapter number | 16 |
Book title |
Plant Organogenesis
|
Published in |
Methods in molecular biology, November 2012
|
DOI | 10.1007/978-1-62703-221-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-62703-220-9, 978-1-62703-221-6
|
Authors |
Ram Kishor Yadav, Stephen Snipes, Thomas Girke, G. Venugopala Reddy |
Editors |
Ive De Smet |
Abstract |
Shoot apical meristems (SAMs) of higher plants harbor a set of stem-cells and provide cells for the development of all the above-ground biomass of plants. Most of the important pattern formation events such as maintenance of stem-cell identity, specification and differentiation of leaf/flower primordia, and temporal control of the transition from vegetative to reproductive program are determined in SAMs. Genetic analysis has revealed molecular and hormonal pathways involved in stem-cell maintenance, organ differentiation, and flowering time. However, limited information is available as to how different pathways interact with each other to function as a network in specifying different cell types and their function. Deciphering gene networks that underlie cell fate transitions requires new approaches aimed at assaying genome-scale expression patterns of genes at a single cell-type resolution. Here we provide details of experimental methods involved in protoplasting of SAM cells, generating cell type-specific gene expression profiles, and analysis platforms for identifying and inferring gene networks. |
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