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Bacterial Regulatory RNA

Overview of attention for book
Cover of 'Bacterial Regulatory RNA'

Table of Contents

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    Book Overview
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    Chapter 1 RNA Extraction from Gram-Positive Bacteria Membrane Vesicles Using a Polymer-Based Precipitation Method
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    Chapter 2 Extraction and Purification of Outer Membrane Vesicles and Their Associated RNAs
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    Chapter 3 Analysis of Phage Regulatory RNAs: Sequencing Library Construction from the Fraction of Small Prokaryotic RNAs Less Than 50 Nucleotides in Length.
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    Chapter 4 Discovering Novel Bacterial Small RNA by RNA-seq Analysis Toolkit ANNOgesic.
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    Chapter 5 Ribosome Profiling Methods Adapted to the Study of RNA-Dependent Translation Regulation in Staphylococcus aureus
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    Chapter 6 CRISPR Interference-Based Functional Small RNA Genomics
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    Chapter 7 Investigation of sRNA-mRNA Interactions in Bacillus subtilis In Vivo
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    Chapter 8 In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis
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    Chapter 9 RNA Double-Helix Hybridization Measured by Fluorescence Correlation Spectroscopy
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    Chapter 10 New Perspectives on Crosstalks Between Bacterial Regulatory RNAs from Outer Membrane Vesicles and Eukaryotic Cells
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    Chapter 11 Experimental Validation of RNA–RNA Interactions by Electrophoretic Mobility Shift Assay
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    Chapter 12 Dynamics and Function of sRNA/mRNAs Under the Scrutiny of Computational Simulation Methods
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    Chapter 13 Analysis of sRNAs and Their mRNA Targets in Sinorhizobium meliloti : Focus on Half-Life Determination
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    Chapter 14 Evaluation of 5′-End Phosphorylation for Small RNA Stability and Target Regulation In Vivo
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    Chapter 15 In-Gel Cyanoethylation for Pseudouridines Mass Spectrometry Detection of Bacterial Regulatory RNA
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    Chapter 16 Directed Screening for sRNA Targets in E. coli Using a Plasmid Library
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    Chapter 17 Defining Bacterial RNA-RNA Interactomes Using CLASH
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    Chapter 18 Global Identification of RNA-Binding Proteins in Bacteria
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    Chapter 19 An Integrated Affinity Chromatography-Based Approach to Unravel the sRNA Interactome in Nitrogen-Fixing Rhizobia
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    Chapter 20 sRNA Structural Modeling Based on NMR Data.
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    Chapter 21 Circular and Linear Dichroism for the Analysis of Small Noncoding RNA Properties
Attention for Chapter 20: sRNA Structural Modeling Based on NMR Data.
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Chapter title
sRNA Structural Modeling Based on NMR Data.
Chapter number 20
Book title
Bacterial Regulatory RNA
Published in
Methods in molecular biology, January 2024
DOI 10.1007/978-1-0716-3565-0_20
Pubmed ID
38217664
Book ISBNs
978-1-07-163564-3, 978-1-07-163565-0
Authors

Wu, Pengzhi, Yang, Lingna

Abstract

Small non-coding RNAs (sRNAs) play vital roles in gene expression regulation and RNA interference. To comprehend their molecular mechanisms and develop therapeutic approaches, determining the accurate three-dimensional structure of sRNAs is crucial. Although nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for structural biology, obtaining high-resolution structures of sRNAs using NMR data alone can be challenging. In such cases, structural modeling can provide additional details about RNA structures. In this context, we present a protocol for the structural modeling of sRNA using the SimRNA method based on sparse NMR constraints. To demonstrate the efficacy of our method, we provide selected examples of NMR spectra and RNA structures, specifically for the second stem-loop of DsrA sRNA.

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 Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2024.
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#20,474,324
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Outputs from Methods in molecular biology
#8,886
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#139,170
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Outputs of similar age from Methods in molecular biology
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