Chapter title |
Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients.
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Book title |
Single Cell Analysis
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Published in |
Methods in molecular biology, January 2024
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DOI | 10.1007/978-1-0716-3621-3_7 |
Pubmed ID | |
Book ISBNs |
978-1-07-163620-6, 978-1-07-163621-3
|
Authors |
Reduzzi, Carolina, Vismara, Marta, Schamberger, Thomas, Silvestri, Marco, Motta, Rosita, Polzer, Bernhard M, Cappelletti, Vera, Carolina Reduzzi, Marta Vismara, Thomas Schamberger, Marco Silvestri, Rosita Motta, Bernhard M. Polzer, Vera Cappelletti, Polzer, Bernhard M. |
Abstract |
The role of circulating tumor cell (CTC) clusters in the metastatic dissemination process is gaining increased attention. Besides homotypic clusters, heterotypic clusters that contain tumor cells admixed with normal cells are frequently observed in patients with solid tumors. Current methods used for cluster detection and enumeration do not allow an accurate estimation of the relative fractions of tumor cells. Here we describe a method for estimating tumor fraction of clusters including isolation and collection of single clusters, assessment of copy number alterations of single clusters by low-pass whole genome sequencing, and bioinformatic analysis of sequencing data. |
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Mendeley readers
Geographical breakdown
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Unknown | 3 | 100% |
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