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Alpha-1 Antitrypsin

Overview of attention for book
Cover of 'Alpha-1 Antitrypsin'

Table of Contents

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    Book Overview
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    Chapter 1 Alpha-1 Antitrypsin Deficiency
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    Chapter 2 Approaches to Therapeutic Gene Editing in Alpha-1 Antitrypsin Deficiency
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    Chapter 3 Manipulation of Proteostasis Networks in Transgenic ZAAT Zebrafish via CRISPR-Cas9 Gene Editing.
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    Chapter 4 PCR Analysis to Identify AAT Gene Promoters and Splice Variants
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    Chapter 5 Native and Ion Mobility Mass Spectrometry Characterization of Alpha 1 Antitrypsin Variants and Oligomers
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    Chapter 6 Sanger and Next-Generation Sequencing of AAT.
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    Chapter 7 Measuring the Concentration of Active Alpha-1 Antitrypsin in Serum
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    Chapter 8 Characterization of Novel Alpha-1-Antitrypsin Coding Variants in a Mammalian Cellular Model
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    Chapter 9 Alpha-1-Antitrypsin (A1AT) Proteotyping by LC-MS/MS
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    Chapter 10 Serum Western Blot for the Detection of a c-Myc Protein Tag in Non-human Primates and Mice
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    Chapter 11 An Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Circulating Pi*Z Alpha1-Antitrypsin Polymers
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    Chapter 12 Measuring of Alpha-1 Antitrypsin Concentration by Nephelometry or Turbidimetry
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    Chapter 13 Autoantibody Recognition of Natural and Homocysteinylated Alpha-1 Antitrypsin: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Quantification in Sera
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    Chapter 14 Monitoring the Secretion and Activity of Alpha-1 Antitrypsin in Various Mammalian Cell Types.
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    Chapter 15 Lipoproteins in Negative Feedback with Alpha-1 Antitrypsin
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    Chapter 16 Silencing Very-Low-Density Lipoprotein Receptor Reveals Alpha-1 Antitrypsin Role in HIV Infectivity
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    Chapter 17 Purified Versus Plasma Alpha-1 Antitrypsin Effects on Cellular Activities
Attention for Chapter 14: Monitoring the Secretion and Activity of Alpha-1 Antitrypsin in Various Mammalian Cell Types.
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Chapter title
Monitoring the Secretion and Activity of Alpha-1 Antitrypsin in Various Mammalian Cell Types.
Chapter number 14
Book title
Alpha-1 Antitrypsin
Published in
Methods in molecular biology, January 2024
DOI 10.1007/978-1-0716-3605-3_14
Pubmed ID
38108975
Book ISBNs
978-1-07-163604-6, 978-1-07-163605-3
Authors

Guay, Kevin P, Ke, Haiping, Gierasch, Lila M, Gershenson, Anne, Hebert, Daniel N, Guay, Kevin P., Gierasch, Lila M., Hebert, Daniel N.

Abstract

Overexpression of recombinant protein in mammalian cells is widely used for producing biologics, as protein maturation and post-translational modifications are similar to human cells. Some therapeutics, such as mRNA vaccines, target nonnative cells that may contain inefficient secretory machinery. For example, gene replacement therapies for alpha-1 antitrypsin (AAT), a glycoprotein normally produced in hepatocytes, are often targeted to muscle cells due to ease of delivery. In this chapter, we define methods for expressing AAT in representative cell types such as Huh-7; hepatocytes; Chinese hamster ovarian cells (CHO), a common host to produce biologics; and C2C12, a muscle progenitor cell line. Methods for metabolically labeling AAT to monitor secretion in these cell lines are described along with the use of proteostasis activators to increase the amount of AAT secreted in both C2C12 myoblasts and differentiated myotubes. Assays to assess the activity and glycan composition of overexpressed AAT are also presented. The usage of the proteostasis activator SAHA provided a 40% improvement in expression of active AAT in muscle-like cells and may be an advantageous adjuvant for recombinant production of proteins delivered by mRNA vaccines.

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Geographical breakdown

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Readers by professional status Count As %
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Readers by discipline Count As %
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Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2023.
All research outputs
#17,024,560
of 25,016,456 outputs
Outputs from Methods in molecular biology
#5,908
of 14,081 outputs
Outputs of similar age
#59,883
of 118,121 outputs
Outputs of similar age from Methods in molecular biology
#77
of 192 outputs
Altmetric has tracked 25,016,456 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,081 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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We're also able to compare this research output to 192 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.

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