Reverse Genetics of RNA Viruses
Springer US
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Attention Score in Context
| Chapter title |
Reverse Genetics of Hepatitis C Virus Using an RNA Polymerase I-Mediated Transcription.
|
|---|---|
| Book title |
Reverse Genetics of RNA Viruses
|
| Published in |
Methods in molecular biology, January 2024
|
| DOI | 10.1007/978-1-0716-3533-9_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-07-163532-2, 978-1-07-163533-9
|
| Authors |
Suzuki, Ryosuke, Suzuki, Tetsuro |
| Abstract |
The reverse genetics system commonly used for the production of hepatitis C virus (HCV), which is a major causative agent of liver diseases, involves introduction of the viral genomic RNA synthesized in vitro into human hepatoma cells by electroporation. As an alternative methodology, we describe a cell culture system based on transfection with an expression plasmid containing a full-length HCV cDNA clone flanked by RNA polymerase I promoter and terminator sequences to generate infectious virus particles from transfected cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2023.
All research outputs
#16,983,610
of 24,965,047 outputs
Outputs from Methods in molecular biology
#5,858
of 14,033 outputs
Outputs of similar age
#42,378
of 85,497 outputs
Outputs of similar age from Methods in molecular biology
#48
of 150 outputs
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