Chapter title |
In Situ Protein Microarray for Identifying the Geminivirus-Arabidopsis Interactome.
|
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Book title |
Plant-Virus Interactions
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Published in |
Methods in molecular biology, January 2024
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DOI | 10.1007/978-1-0716-3485-1_22 |
Pubmed ID | |
Book ISBNs |
978-1-07-163484-4, 978-1-07-163485-1
|
Authors |
Yazaki, Junshi, Dal-Bianco, Maximiller |
Abstract |
The protein-protein interactions (PPI) by protein array technology complement other PPI assay technologies such as AP-MS and Y2H. The in situ protein array technology (NAPPA) enables low-cost, rapid, and comprehensive protein detection. It allows standardized and simultaneous assay of a wide range of proteins with a broad range of expression in cells. This technology facilitates the detection of protein-protein interactions within species and between heterologous species such as host-microbe. Here, we described the technique that identified a syntaxin-6 protein-mediated begomovirus infection using an array containing 4600 Arabidopsis genes. The protein microarray assay also identified several other viral protein-host protein interactions. |
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