Plant-Virus Interactions
Springer US
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Attention Score in Context
| Chapter title |
Construction of Geminivirus Infectious Clones for Agroinoculation into Plants.
|
|---|---|
| Book title |
Plant-Virus Interactions
|
| Published in |
Methods in molecular biology, January 2024
|
| DOI | 10.1007/978-1-0716-3485-1_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-07-163484-4, 978-1-07-163485-1
|
| Authors |
Morais, Ivair J, Inoue-Nagata, Alice K, Nakasu, Erich Y T, Morais, Ivair J., Inoue-Nagata, Alice K., Nakasu, Erich Y. T. |
| Abstract |
The production of geminiviral infectious clones provides a standardized inoculum for use in several host-virus studies. Geminiviruses present either one (monopartite) or two (bipartite) circular single-stranded DNA components, which commonly range from 2.6 to 2.8 kb. Cloning of a monomeric genome is useful for obtaining its precise sequence. For infectious clones, however, it is essential that more than one copy of the genome, more specifically of the origin of replication, is present in order to guarantee the production of full-length progeny DNA. Here, the complete process of preparing infectious geminiviral clones is described starting from the DNA extraction and selection of restriction endonucleases followed by two protocols for constructing dimeric clones: restriction endonuclease digestion and ligation (1) and Gibson Assembly (2). |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2023.
All research outputs
#15,166,695
of 25,380,192 outputs
Outputs from Methods in molecular biology
#4,108
of 14,200 outputs
Outputs of similar age
#129,032
of 324,914 outputs
Outputs of similar age from Methods in molecular biology
#45
of 274 outputs
Altmetric has tracked 25,380,192 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,200 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 324,914 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 274 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.