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Pseudomonas aeruginosa

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Cover of 'Pseudomonas aeruginosa'

Table of Contents

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    Book Overview
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    Chapter 1 CRISPR/Cas9-based Genome Editing of Pseudomonas aeruginosa.
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    Chapter 2 Investigating Pseudomonas aeruginosa Gene Function During Pathogenesis Using Mobile-CRISPRi
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    Chapter 3 Engineering Green-light-responsive Heterologous Gene Expression in Pseudomonas.
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    Chapter 5 Whole-Cell Biosensors for Qualitative and Quantitative Analysis of Quorum Sensing Signal Molecules and the Investigation of Quorum Quenching Agents.
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    Chapter 6 A Pseudomonas aeruginosa -Suitable Fluorescent Reporter System for Analyzing Small RNA-Mediated Regulation of Target mRNAs
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    Chapter 7 The Pseudomonas aeruginosa Resistome: Permanent and Transient Antibiotic Resistance, an Overview
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    Chapter 8 Biosensors for Inducers of Transient Antibiotic Resistance
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    Chapter 9 Pseudomonas aeruginosa Soluble Pyocins as Antibacterial Weapons
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    Chapter 10 Assays for Studying Pseudomonas aeruginosa Secreted Proteases
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    Chapter 11 Single Microcolony Diffusion Analysis in Pseudomonas aeruginosa Biofilms
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    Chapter 12 Broad Genome Sequencing of Environmental and Clinical Strains and Genotyping
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    Chapter 13 Genome-Scale Analysis of the Structure and Function of RNA Pathways and Networks in Pseudomonas aeruginosa
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    Chapter 14 In-Depth Quantitative Proteomics Analysis of the Pseudomonas aeruginosa Secretome
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    Chapter 15 Improving the Predictive Value of Preclinical Mouse Models of Pseudomonas aeruginosa Respiratory Infection to Evaluate Antibiotic Efficacy
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    Chapter 16 Emerging In Vitro Models for the Study of Infection and Pathogenesis of Pseudomonas aeruginosa and Testing of Antibacterial Agents.
Attention for Chapter: Fluorescence-based Evaluation of Cyclic di-GMP Levels in Pseudomonas aeruginosa.
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Chapter title
Fluorescence-based Evaluation of Cyclic di-GMP Levels in Pseudomonas aeruginosa.
Book title
Pseudomonas aeruginosa
Published in
Methods in molecular biology, January 2024
DOI 10.1007/978-1-0716-3473-8_4
Pubmed ID
Book ISBNs
978-1-07-163472-1, 978-1-07-163473-8
Authors

Santoro, Silvia, Bertoni, Giovanni, Ferrara, Silvia

Abstract

The ability of Pseudomonas aeruginosa to establish chronic infections is associated with an effective switch from a motile to a sessile lifestyle. This proficiency is controlled by intracellular levels of the second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP). Targeting the c-di-GMP network could be a strategy to interfere with P. aeruginosa pathogenicity. Therefore, the development of tools to profile c-di-GMP intracellular levels is crucial. Here, we describe a protocol for the in vivo measurement of c-di-GMP levels in P. aeruginosa.

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Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2023.
All research outputs
#19,332,255
of 24,615,949 outputs
Outputs from Methods in molecular biology
#7,976
of 13,850 outputs
Outputs of similar age
#7,086
of 9,938 outputs
Outputs of similar age from Methods in molecular biology
#23
of 38 outputs
Altmetric has tracked 24,615,949 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,850 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
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We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.