CD95
Springer New York
Chapter title |
Organelle Separation and Cell Signaling
|
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Chapter number | 11 |
Book title |
CD95
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6780-3_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6778-0, 978-1-4939-6780-3
|
Authors |
Leandro Silva Da Costa, Damien Arnoult |
Editors |
Patrick Legembre |
Abstract |
Recent findings indicate that some signaling hubs coalesce at the surfaces of organelles through the accumulation of ubiquitylated components required for the signal transduction. For instance, ubiquitylated components of the NF-κB pathway accumulated at the endoplasmic reticulum while ubiquitylated components of the IRF3 pathway are found at the Golgi apparatus. Here we describe simple methods to observe and assess these ubiquitylated components by immunoblotting using differential centrifugation and in vitro assays. |
Country | Count | As % |
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Unknown | 3 | 100% |
Readers by professional status | Count | As % |
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Researcher | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 1 | 33% |
Unknown | 2 | 67% |