Nipah Virus
Springer US
Chapter title |
A Revised Diagnostic Quantitative RT-PCR for the Detection of Nipah Virus Infection.
|
---|---|
Chapter number | 2 |
Book title |
Nipah Virus
|
Published in |
Methods in molecular biology, January 2023
|
DOI | 10.1007/978-1-0716-3283-3_2 |
Pubmed ID | |
Book ISBNs |
978-1-07-163282-6, 978-1-07-163283-3
|
Authors |
Patel, Ketan, Klena, John, Lo, Michael K, Lo, Michael K. |
Abstract |
From its discovery in Malaysia in the late 1990s, the spillover of the Nipah virus from its pteropid reservoir into the human population has resulted in sporadic outbreaks of fatal encephalitis and respiratory disease. In this chapter, we revise a previously described quantitative reverse transcription polymerase chain reaction method, which now utilizes degenerate nucleotides at certain positions in the probe and the reverse primer to accommodate the sequence heterogeneity observed within the Nipah henipavirus species. |
Country | Count | As % |
---|---|---|
United States | 2 | 10% |
Spain | 1 | 5% |
France | 1 | 5% |
Unknown | 16 | 80% |
Type | Count | As % |
---|---|---|
Members of the public | 18 | 90% |
Scientists | 2 | 10% |
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 50% |
Researcher | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 50% |
Medicine and Dentistry | 1 | 25% |
Unknown | 1 | 25% |