Chaperones
Springer US
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| Chapter title |
Complementation Assays for Co-chaperone Function.
|
|---|---|
| Book title |
Chaperones
|
| Published in |
Methods in molecular biology, January 2023
|
| DOI | 10.1007/978-1-0716-3342-7_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-07-163341-0, 978-1-07-163342-7
|
| Authors |
Edkins, Adrienne L, Blatch, Gregory L, Adrienne L. Edkins, Gregory L. Blatch, Edkins, Adrienne L., Blatch, Gregory L. |
| Abstract |
The development of mutant microorganisms lacking J domain proteins (JDPs; formerly called Hsp40s) has enabled the development of complementation assays for testing the co-chaperone function of JDPs. In these assays, an exogenously expressed novel JDP is tested for its ability to functionally substitute for a non-expressed or nonfunctional endogenous JDP(s) by reversing a stress phenotype. For example, the in vivo functionality of prokaryotic JDPs can be tested on the basis of their ability to reverse the thermosensitivity of a dnaJ cbpA mutant strain of the bacterium Escherichia coli (OD259). Similarly, the in vivo functionality of eukaryotic JDPs can be assessed in a thermosensitive ydj1 mutant strain of the yeast Saccharomyces cerevisiae (JJ160). Here we outline the use of these thermosensitive microorganisms in complementation assays to functionally characterize a JDP from the bacterium, Agrobacterium tumefaciens (AgtDnaJ), and a JDP from the trypanosomal parasite, Trypanosoma cruzi (TcJ2). |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 August 2023.
All research outputs
#20,587,875
of 25,295,968 outputs
Outputs from Methods in molecular biology
#8,925
of 14,174 outputs
Outputs of similar age
#350,048
of 476,778 outputs
Outputs of similar age from Methods in molecular biology
#511
of 722 outputs
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