| Chapter title |
Discovery and Subtyping of Neo-Epitope Specific T-Cell Responses for Cancer Immunotherapy: Addressing the Mutanome.
|
|---|---|
| Chapter number | 14 |
| Book title |
RNA Vaccines
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6481-9_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6479-6, 978-1-4939-6481-9
|
| Authors |
Mustafa Diken, Mathias Vormehr, Christian Grunwitz, Sebastian Kreiter, Özlem Türeci, Ugur Sahin |
| Editors |
Thomas Kramps, Knut Elbers |
| Abstract |
Cancer accumulates 10s to 1000s of genomic mutations of which a fraction is immunogenic and may serve as an Achilles' heel of tumor cells. Mutation-specific T cells can recognize these antigens and destroy malignant cells. Strategies to immunotherapeutically address individual tumor mutations employing peptide or mRNA based vaccines are now actively investigated in mice and humans. An important step of determining the therapeutic potential of a mutanome vaccine is the detection of mutation reactive T-cell responses. In this chapter we provide protocols to identify and subtype mutation specific T cells in mice based on IFN-γ ELISpot and flow cytometry. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 17% |
| Student > Master | 6 | 17% |
| Researcher | 4 | 11% |
| Professor | 4 | 11% |
| Other | 2 | 6% |
| Other | 4 | 11% |
| Unknown | 9 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 7 | 20% |
| Agricultural and Biological Sciences | 6 | 17% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 9% |
| Chemistry | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 12 | 34% |