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Synapse Development

Overview of attention for book
Cover of 'Synapse Development'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 A Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction.
  3. Altmetric Badge
    Chapter 2 Co-culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices.
  4. Altmetric Badge
    Chapter 3 Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates.
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    Chapter 4 Monitoring Synapses Via Trans-Synaptic GFP Complementation.
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    Chapter 5 Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells.
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    Chapter 6 Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins.
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    Chapter 7 In Situ Protein Binding Assay Using Fc-Fusion Proteins.
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    Chapter 8 Reconstitution of Synaptic SNAREs into Large Liposomes with Reduced Curvature Stress.
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    Chapter 9 Isolation of Synaptosomes, Synaptic Plasma Membranes, and Synaptic Junctional Complexes.
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    Chapter 10 Purification of Synaptosome Populations Using Fluorescence-Activated Synaptosome Sorting.
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    Chapter 11 Optimized Protocol for Imaging Cleared Neural Tissues Using Light Microscopy.
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    Chapter 12 Structured Illumination Microscopy for the Investigation of Synaptic Structure and Function.
  14. Altmetric Badge
    Chapter 13 3D d STORM Imaging of Fixed Brain Tissue.
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    Chapter 14 Synapse Development
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    Chapter 15 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography.
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    Chapter 16 Analyzing Endosomal Docking, Fusion, Sorting, and Budding Mechanisms in Isolated Organelles.
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    Chapter 17 Concurrent Imaging of Receptor Trafficking and Calcium Dynamics by Spinning Disk Confocal Microscopy.
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    Chapter 18 Imaging Activity-Dependent Signaling Dynamics at the Neuronal Synapse Using FRET-Based Biosensors.
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    Chapter 19 Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture.
  21. Altmetric Badge
    Chapter 20 Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ.
  22. Altmetric Badge
    Chapter 21 Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity.
  23. Altmetric Badge
    Chapter 22 Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging.
  24. Altmetric Badge
    Chapter 23 Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.
Attention for Chapter 14: Synapse Development
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Chapter title
Synapse Development
Chapter number 14
Book title
Synapse Development
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6688-2_14
Pubmed ID
Book ISBNs
978-1-4939-6686-8, 978-1-4939-6688-2
Authors

Bosch, Miquel, Castro, Jorge, Sur, Mriganka, Hayashi, Yasunori, Miquel Bosch, Jorge Castro, Mriganka Sur, Yasunori Hayashi

Editors

Alexandros Poulopoulos

Abstract

Synapses learn and remember by persistent modifications of their internal structures and composition but, due to their small size, it is difficult to observe these changes at the ultrastructural level in real time. Two-photon fluorescence microscopy (2PM) allows time-course live imaging of individual synapses but lacks ultrastructural resolution. Electron microscopy (EM) allows the ultrastructural imaging of subcellular components but cannot detect fluorescence and lacks temporal resolution. Here, we describe a combination of procedures designed to achieve the correlated imaging of the same individual synapse under both 2PM and EM. This technique permits the selective stimulation and live imaging of a single dendritic spine and the subsequent localization of the same spine in EM ultrathin serial sections. Landmarks created through a photomarking method based on the 2-photon-induced precipitation of an electrodense compound are used to unequivocally localize the stimulated synapse. This technique was developed to image, for the first time, the ultrastructure of the postsynaptic density in which long-term potentiation was selectively induced just seconds or minutes before, but it can be applied for the study of any biological process that requires the precise relocalization of micron-wide structures for their correlated imaging with 2PM and EM.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 33%
Professor 2 22%
Student > Bachelor 1 11%
Librarian 1 11%
Student > Ph. D. Student 1 11%
Other 1 11%
Readers by discipline Count As %
Neuroscience 5 56%
Agricultural and Biological Sciences 2 22%
Biochemistry, Genetics and Molecular Biology 1 11%
Medicine and Dentistry 1 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 December 2016.
All research outputs
#15,404,272
of 22,914,829 outputs
Outputs from Methods in molecular biology
#5,360
of 13,131 outputs
Outputs of similar age
#256,369
of 420,479 outputs
Outputs of similar age from Methods in molecular biology
#466
of 1,074 outputs
Altmetric has tracked 22,914,829 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.