Report for: Antifungal Immunity

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Chapter title	Quantifying the Mechanical Properties of Yeast Candida albicans Using Atomic Force Microscopy-based Force Spectroscopy.
Chapter number	1
Book title	Antifungal Immunity
Published in	Methods in molecular biology, January 2023
DOI	10.1007/978-1-0716-3199-7_1
Pubmed ID	37145272
Book ISBNs	978-1-07-163198-0, 978-1-07-163199-7
Authors	Jones, Christopher R, Zhang, Zhenyu Jason, Tsai, Hung-Ji, Jones, Christopher R.
Abstract	Fungi can adapt to a wide range of environmental stresses in the wild and host milieu by employing their plastic genome and great diversity in morphology. Among different adaptive strategies, mechanical stimuli, such as changes in osmotic pressure, surface remodeling, hyphal formation, and cell divisions, could guide the physical cues into physiological responses through a complex signaling network. While fungal pathogens require a pressure-driven force to expand and penetrate host tissues, quantitatively studying the biophysical properties at the host-fungal interface is critical to understand the development of fungal diseases. Microscopy-based techniques have enabled researchers to monitor the dynamic mechanics on fungal cell surface in responses to the host stress and antifungal drugs. Here, we describe a label-free, high-resolution method based on atomic force microscopy, with a step-by-step protocol to measure the physical properties in human fungal pathogen Candida albicans.

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This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2023.

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Antifungal Immunity

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