Report for: Advanced Methods in Structural Biology

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Chapter title	Fluorescent Fusion Protein Expression in Plant Cells.
Book title	Advanced Methods in Structural Biology
Published in	Methods in molecular biology, January 2023
DOI	10.1007/978-1-0716-3147-8_6
Pubmed ID	37093472
Book ISBNs	978-1-07-163146-1, 978-1-07-163147-8
Authors	Huang, Shuxian, Yonglun, Zeng
Abstract	Fluorescent proteins (FPs) revolutionized the cell biology research by visualizing the dynamics of cellular events. In fusion with the targeted proteins, the FPs can be utilized to monitor the protein dynamics and localization in cells. Recently, FPs have been used as reporters for live cell imaging to study the protein localization or organelles dynamics in plants, allowing cell biologists to explore the plant cell function by obtaining tremendous details of cell structures and functions in combination with confocal imaging. To facilitate the usage of fluorescent proteins for protein localization and dynamic analysis in plant cell biology research, here we describe the updated protocol of Agrobacterium-mediated transformation of Arabidopsis thaliana using fluorescent proteins to generate the stable expression transgenic plants for protein trafficking and localization study. We further use the GFP-tagged SDP1 (sugar-dependent protein) lipase, mCherry-tagged peroxisome marker, and BODYPY or Nile Red (lipid droplet staining dye) as examples to introduce the method for the protein localization analysis in plants.

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Advanced Methods in Structural Biology

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