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Heterologous Expression of Membrane Proteins

Overview of attention for book
Cover of 'Heterologous Expression of Membrane Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Solubilization and Purification of α5β1 Integrin from Rat Liver for Reconstitution into Nanodiscs
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    Chapter 2 Membrane Protein Production in Escherichia coli : Protocols and Rules
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    Chapter 3 Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly
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    Chapter 4 Heterologous Expression of Membrane Proteins in E. coli
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    Chapter 5 Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli
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    Chapter 6 Production of Membrane Proteins in Pseudomonas stutzeri
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    Chapter 7 Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae
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    Chapter 8 Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae
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    Chapter 9 Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p
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    Chapter 10 Membrane Protein Production in the Yeast Yeasts P. pastoris
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    Chapter 11 Production and Preparation of Isotopically Labeled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses Fast-MAS-NMR analyses
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    Chapter 12 Membrane Protein Production in Insect Cells
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    Chapter 13 Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies
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    Chapter 14 Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies
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    Chapter 15 Heterologous Expression and Purification of GPCRs
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    Chapter 16 Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor
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    Chapter 17 Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-like Particles
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    Chapter 18 Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface
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    Chapter 19 Tailoring Purification and Analysis of Membrane Proteins with Modular Detergents
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    Chapter 20 Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers
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    Chapter 21 Detergent-Free Membrane Protein Purification Using SMA Polymer
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    Chapter 22 Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies
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    Chapter 23 Cell-Free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs Nanodiscs
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    Chapter 24 Microscale Thermophoresis to Evaluate the Functionality of Heterologously Overexpressed Membrane Proteins in Membrane Preparations
Attention for Chapter 10: Membrane Protein Production in the Yeast Yeasts P. pastoris
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Chapter title
Membrane Protein Production in the Yeast Yeasts P. pastoris
Chapter number 10
Book title
Heterologous Expression of Membrane Proteins
Published in
Methods in molecular biology, January 2022
DOI 10.1007/978-1-0716-2368-8_10
Pubmed ID
Book ISBNs
978-1-07-162367-1, 978-1-07-162368-8
Authors

Ayub, Hoor, Clare, Michelle, Broadbent, Luke, Simms, John, Goddard, Alan D., Rothnie, Alice J., Bill, Roslyn M.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 July 2022.
All research outputs
#15,812,910
of 23,485,953 outputs
Outputs from Methods in molecular biology
#5,367
of 12,978 outputs
Outputs of similar age
#15,470,660
of 22,924,003 outputs
Outputs of similar age from Methods in molecular biology
#5,381
of 12,971 outputs
Altmetric has tracked 23,485,953 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 12,978 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 12,971 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.