Dendritic Cells
Springer US
Chapter title |
Characterization of Developmental Trajectories of Dendritic Cell Hematopoiesis Through Single-Cell RNA Sequencing Methods.
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Chapter number | 23 |
Book title |
Dendritic Cells
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Published in |
Methods in molecular biology, January 2023
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DOI | 10.1007/978-1-0716-2938-3_23 |
Pubmed ID | |
Book ISBNs |
978-1-07-162937-6, 978-1-07-162938-3
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Authors |
Dress, Regine J, Ginhoux, Florent, Dress, Regine J. |
Abstract |
Single-cell technologies have become valuable tools to trace dendritic cell differentiation trajectories. Here, we illustrate the workflow used for processing of mouse bone marrow for single-cell RNA sequencing and trajectory analyses, as done in Dress et al. (Nat Immunol 20:852-864, 2019). This short methodology is presented as a starting point for researchers just beginning to dive into the complex field of dendritic cell ontogeny and cellular development trajectory analyses. |
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