Z-DNA
Springer US
Chapter title |
Crystallization of Z-DNA in Complex with Chemical and Z-DNA Binding Z-Alpha Protein.
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Chapter number | 4 |
Book title |
Z-DNA
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Published in |
Methods in molecular biology, January 2023
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DOI | 10.1007/978-1-0716-3084-6_4 |
Pubmed ID | |
Book ISBNs |
978-1-07-163083-9, 978-1-07-163084-6
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Authors |
Kang, MinSoung, Kim, Doyoun |
Abstract |
The molecular basis of Z-DNA recognition and stabilization is mostly discovered via X-ray crystallography. The sequences composed with alteration of purine and pyrimidine are known to adopt Z-DNA conformation. Due to the energy penalty for forming Z-DNA, the small molecular stabilizer or Z-DNA-specific binding protein is required for DNA to adopt Z conformation prior to crystallizing Z-DNA. Here we described the methods ranging from preparation of DNA and Z-alpha protein to crystallization of Z-DNA in detail. |
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