| Chapter title |
Quantification of intracellular mitochondrial displacements in response to nanomechanical forces.
|
|---|---|
| Chapter number | 18 |
| Book title |
Cellular and Subcellular Nanotechnology
|
| Published in |
Methods in molecular biology, April 2013
|
| DOI | 10.1007/978-1-62703-336-7_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-335-0, 978-1-62703-336-7
|
| Authors |
Silberberg YR, Pelling AE, Yaron R. Silberberg, Andrew E. Pelling, Silberberg, Yaron R., Pelling, Andrew E. |
| Abstract |
Mechanical stress affects various aspects of cell behavior, including cell growth, morphology, differentiation, and genetic expression. Here, we describe a method to quantify the intracellular mechanical response to an extracellular mechanical perturbation, specifically the displacement of mitochondria. A combined fluorescent-atomic force microscope (AFM) was used to simultaneously produce well-defined nanomechanical stimulation to a living cell while optically recording the real-time displacement of fluorescently labeled mitochondria. A single-particle tracking (SPT) approach was then applied in order to quantify the two-dimensional displacement of mitochondria in response to local forces. |
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Mendeley readers
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