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Chapter title |
Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research
|
---|---|
Chapter number | 17 |
Book title |
Antibiotics
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6634-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6632-5, 978-1-4939-6634-9
|
Authors |
Florian Bonn, Sandra Maass, Dörte Becher |
Editors |
Peter Sass |
Abstract |
Absolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of multi-protein complexes. In this chapter, a universal protocol for gel free absolute protein quantification in bacterial systems is described, which can be used for sample preparation prior to miscellaneous mass-spectrometry-based quantification workflows like AQUA, Hi3, and emPAI. In addition, a focus has been set to the specific challenges in antibiotic stress research. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 1 | 14% |
Student > Bachelor | 1 | 14% |
Student > Master | 1 | 14% |
Researcher | 1 | 14% |
Professor > Associate Professor | 1 | 14% |
Other | 0 | 0% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Computer Science | 2 | 29% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Engineering | 1 | 14% |
Unknown | 2 | 29% |