Protein Aggregation

Table of Contents

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   Book Overview
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   Chapter 1 Early Aggregation of Amyloid-β(1–42) Studied by Fluorescence Correlation Spectroscopy
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   Chapter 2 Preparation and Investigation of Crucial Oligomers in the Early Stages of Aβ40 and Aβ42 Aggregation
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   Chapter 3 Preparation and Fractionation of Heterogeneous Aβ42 Oligomers with Different Aggregation Properties
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   Chapter 4 An Efficient Method of Expression and Purification of Amyloid-Beta (Aβ1–42) Peptide from E. coli
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   Chapter 5 Solid-State NMR Structure of Amyloid-β Fibrils
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   Chapter 6 Time-Resolved In Situ AFM Measurement of Growth Rates of Aβ40 Fibrils
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   Chapter 7 Monitoring Kinetics of pH-Dependent Aggregation and Disaggregation of the Pmel17 Repeat Domain
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   Chapter 8 Analysis of Tau/Nucleoporin Interactions by Surface Plasmon Resonance Spectroscopy
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    Chapter 9 Microfluidic Chamber Technology to Study Missorting and Spreading of Tau Protein in Alzheimer’s Disease
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    Chapter 10 Using FRET-Based Biosensor Cells to Study the Seeding Activity of Tau and α-Synuclein
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    Chapter 11 Functional Applications of Stable Tau Oligomers in Cell Biology and Electrophysiology Studies
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    Chapter 12 An Additive-Free Model for Tau Self-Assembly
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    Chapter 13 Cross-Linking Mass Spectrometry Analysis of Metastable Compact Structures in Intrinsically Disordered Proteins
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    Chapter 14 A Validated Method to Prepare Stable Tau Oligomers
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    Chapter 15 Light Microscopy and Dynamic Light Scattering to Study Liquid-Liquid Phase Separation of Tau Proteins In Vitro
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    Chapter 16 Study of Tau Liquid-Liquid Phase Separation In Vitro
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    Chapter 17 Liquid-Liquid Phase Separation to Study the Association of Proteins in Solution
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    Chapter 18 Mapping Phase Diagram of Tau-RNA LLPS Under Live Cell Coculturing Conditions
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    Chapter 19 The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism: A Methodological Approach
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    Chapter 20 Reproducible Formation of Insulin Superstructures: Amyloid-Like Fibrils, Spherulites, and Particulates
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    Chapter 21 CD and Solid-State NMR Studies of Low-Order Oligomers of Transthyretin
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    Chapter 22 Identifying Biological and Biophysical Features of Different Maturation States of α-Synuclein Amyloid Fibrils
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    Chapter 23 α-Synuclein Fibril, Ribbon and Fibril-91 Amyloid Polymorphs Generation for Structural Studies
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    Chapter 24 Propagation of Distinct α-Synuclein Strains Within Human Reconstructed Neuronal Network and Associated Neuronal Dysfunctions
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    Chapter 25 Single-Particle Analysis of the Interaction Between Molecules and Protein Aggregated Species by Dual-Color Time-Resolved Fluorescence Spectroscopy
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    Chapter 26 FRAP and FRET Investigation of α-Synuclein Fibrillization via Liquid-Liquid Phase Separation In Vitro and in HeLa Cells
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    Chapter 27 Spectrally Resolved FRET Microscopy of α-Synuclein Phase-Separated Liquid Droplets
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    Chapter 28 Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells
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    Chapter 29 Identification of Distinct Soluble States During Fibril Formation Using Multilinear Analysis of NMR Diffusion Data
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    Chapter 30 Structural Analysis of SOD1 Fibrils with Mass Spectrometry, Limited Proteolysis, and Atomic Force Microscopy (AFM)
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    Chapter 31 Biophysical Studies of LLPS and Aggregation of TDP-43 LCD
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    Chapter 32 A Spectrophotometric Turbidity Assay to Study Liquid-Liquid Phase Separation of UBQLN2 In Vitro
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    Chapter 33 An Optimized Stress Granule Detection Method: Investigation of UBQLN2 Effect on Stress Granule Formation
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    Chapter 34 Neuronal Puncta/Aggregate Formation by WT and Mutant UBQLN2
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    Chapter 35 In Vivo Analysis of a Biomolecular Condensate in the Nervous System of C. elegans
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    Chapter 36 FLIM-FRET Investigation of Heterogeneous Huntingtin Aggregation in HeLa Cells
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    Chapter 37 In Vitro Characterization of Protein:Nucleic Acid Liquid–Liquid Phase Separation by Microscopy Methods and Nanoparticle Tracking Analysis
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    Chapter 38 Cross-Seeding Assay in the Investigation of the Amyloid Core of Prion Fibrils
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    Chapter 39 Mapping the Domain Structure and Aggregation Propensity of Proteins Using a Gateway Plasmid Vector System