Salamanders
Springer US
Chapter title |
A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.
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Chapter number | 22 |
Book title |
Salamanders
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Published in |
Methods in molecular biology, January 2023
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DOI | 10.1007/978-1-0716-2659-7_22 |
Pubmed ID | |
Book ISBNs |
978-1-07-162658-0, 978-1-07-162659-7
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Authors |
Sousounis, Konstantinos, Courtemanche, Katharine, Whited, Jessica L, Whited, Jessica L. |
Abstract |
Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function. |
Country | Count | As % |
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Unknown | 5 | 100% |
Readers by professional status | Count | As % |
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Researcher | 2 | 40% |
Unknown | 3 | 60% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Unknown | 3 | 60% |