| Chapter title |
Profiling of drug action using reporter mice and molecular imaging.
|
|---|---|
| Chapter number | 5 |
| Book title |
Mouse Models for Drug Discovery
|
| Published in |
Methods in molecular biology, December 2009
|
| DOI | 10.1007/978-1-60761-058-8_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-60761-057-1, 978-1-60761-058-8
|
| Authors |
Rando G, Biserni A, Ciana P, Maggi A, Gianpaolo Rando, Andrea Biserni, Paolo Ciana, Adriana Maggi, Rando, Gianpaolo, Biserni, Andrea, Ciana, Paolo, Maggi, Adriana |
| Abstract |
Reporter mice associated to molecular imaging represent a major asset for the study of the spatio-temporal effects of drugs in living animals. The field is still relatively young and so far the number of animals genetically modified to express a given reporter gene ubiquitously and under the control of specific drugs is still limited. For a reporter animal the indispensable elements for the application to drug research and development are (i) the short life of the reporter enabling to have a clear view of the onset as well as the termination of drug effects, (ii) the generalized, drug-dependent activation of the reporter, and (iii) imaging modality suitable for high-throughput analysis. Because of its relative cheapness and ease to perform, in addition to all the above considerations, bioluminescence-based imaging is now regarded as the best imaging technology to be applied to the field of drug research. We show here the application of reporter mouse systems for drug screening in living animals in order to compare drug potency on target and specificity of action. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 15% |
| Switzerland | 1 | 8% |
| Unknown | 10 | 77% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 31% |
| Student > Ph. D. Student | 3 | 23% |
| Professor > Associate Professor | 2 | 15% |
| Professor | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 54% |
| Medicine and Dentistry | 4 | 31% |
| Chemistry | 1 | 8% |
| Computer Science | 1 | 8% |