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T-Cell Differentiation

Overview of attention for book
Cover of 'T-Cell Differentiation'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 The Vast Universe of T Cell Diversity: Subsets of Memory Cells and Their Differentiation
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    Chapter 2 Basic Aspects of T Helper Cell Differentiation
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    Chapter 3 FACS Analysis of Memory T Lymphocytes
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    Chapter 4 Intravital Microscopy Analysis of Hepatic T Cell Dynamics
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    Chapter 5 Analysis of T Cell Activation by Confocal Microscopy
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    Chapter 6 Phenotypic and Functional Analysis of Antigen-Specific T Cell Exhaustion
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    Chapter 7 pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel
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    Chapter 8 Differentiation of Diverse Progenies of Memory T Cells from Naïve CD8+ T Cell Precursors
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    Chapter 9 Gammaretroviral Production and T Cell Transduction to Genetically Retarget Primary T Cells Against Cancer
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    Chapter 10 Measuring Telomerase Activity in Senescent Human T Cells Upon Genetic Modification
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    Chapter 11 Strategies for T Helper Cell Subset Differentiation from Naïve Precursors
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    Chapter 12 Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells
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    Chapter 13 Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation
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    Chapter 14 T-Cell Differentiation
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    Chapter 15 T-Cell Differentiation
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    Chapter 16 Single-Cell RNA Sequencing of Human T Cells
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    Chapter 17 Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry
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    Chapter 18 Developmental and Functional Assays to Study Murine and Human γδ T Cells
Attention for Chapter 15: T-Cell Differentiation
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Chapter title
T-Cell Differentiation
Chapter number 15
Book title
T-Cell Differentiation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6548-9_15
Pubmed ID
Book ISBNs
978-1-4939-6546-5, 978-1-4939-6548-9
Authors

Dominguez, Maria, Roederer, Mario, Chattopadhyay, Pratip K, Maria Dominguez, Mario Roederer, Pratip K. Chattopadhyay

Editors

Enrico Lugli

Abstract

Recently, technologies have been developed to measure expression of 96 (or more) mRNA transcripts at once from a single cell. Here we describe methods and important considerations for use of Fluidigm's BioMark platform for multiplexed single cell gene expression. We describe how to qualify primer/probes, select genes to examine in 96-parameter panels, perform the reverse transcription/cDNA synthesis step, and operate the instrument. In addition, we describe data analysis considerations. This technology has enormous value for characterizing the heterogeneity of T-cells, thereby providing a useful tool for immune monitoring.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 33%
Unknown 2 67%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Unknown 2 67%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 October 2016.
All research outputs
#15,390,684
of 22,896,955 outputs
Outputs from Methods in molecular biology
#5,354
of 13,135 outputs
Outputs of similar age
#256,313
of 420,429 outputs
Outputs of similar age from Methods in molecular biology
#465
of 1,074 outputs
Altmetric has tracked 22,896,955 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,135 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.