Chapter title |
T-Cell Differentiation
|
---|---|
Chapter number | 15 |
Book title |
T-Cell Differentiation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6548-9_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6546-5, 978-1-4939-6548-9
|
Authors |
Dominguez, Maria, Roederer, Mario, Chattopadhyay, Pratip K, Maria Dominguez, Mario Roederer, Pratip K. Chattopadhyay |
Editors |
Enrico Lugli |
Abstract |
Recently, technologies have been developed to measure expression of 96 (or more) mRNA transcripts at once from a single cell. Here we describe methods and important considerations for use of Fluidigm's BioMark platform for multiplexed single cell gene expression. We describe how to qualify primer/probes, select genes to examine in 96-parameter panels, perform the reverse transcription/cDNA synthesis step, and operate the instrument. In addition, we describe data analysis considerations. This technology has enormous value for characterizing the heterogeneity of T-cells, thereby providing a useful tool for immune monitoring. |
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