Chapter 1 A Guide to Using STITCHER for Overlapping Assembly PCR Applications.

Chapter 2 Synthetic Gene Design Using Codon Optimization On-Line (COOL).

Chapter 3 Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering.

Chapter 4 Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli.

Chapter 5 Synthetic DNA

Chapter 6 BASIC: A Simple and Accurate Modular DNA Assembly Method.

Chapter 7 Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides.

Chapter 8 Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).

Chapter 9 PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.

Chapter 10 The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis.

Chapter 11 Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.

Chapter 12 Generation of DNA Constructs Using the Golden GATEway Cloning Method.

Chapter 13 Synthetic DNA

Chapter 14 Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR).

Chapter 15 Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions.

Chapter 16 Synthetic DNA

Chapter 18 Selection of Error-Less Synthetic Genes in Yeast.