Chapter title |
cfDNA-Based NGS IG Analysis in Lymphoma
|
---|---|
Book title |
Immunogenetics
|
Published in |
Methods in molecular biology, January 2022
|
DOI | 10.1007/978-1-0716-2115-8_7 |
Pubmed ID | |
Book ISBNs |
978-1-07-162114-1, 978-1-07-162115-8
|
Authors |
Pott, Christiane, Kotrova, Michaela, Darzentas, Nikos, Brüggemann, Monika, Khouja, Mouhamad, , , Christiane Pott, Michaela Kotrova, Nikos Darzentas, Monika Brüggemann, Mouhamad Khouja |
Abstract |
Liquid biopsy is a novel diagnostic approach at first developed to characterize the molecular profile of solid tumors by analyzing body fluids. For cancer patients, it represents a noninvasive way to monitor the status of the solid tumor with respect to representative biomarkers. There is growing interest in the utilization of circulating tumor DNA (ctDNA) analysis also in the diagnostic and prognostic fields of lymphomas. Clonal immunoglobulin (IG) gene rearrangements are fingerprints of the respective lymphoid malignancy and thus are highly suited as specific molecular targets for minimal residual disease (MRD) detection. Tracing of the clonal IG rearrangement patterns in ctDNA pool during treatment can be used for MRD assessment in B-cell lymphomas. Here, we describe a reproducible next-generation sequencing assay to identify and characterize clonal IG gene rearrangements for MRD detection in cell-free DNA. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 20% |
Other | 2 | 20% |
Student > Postgraduate | 1 | 10% |
Unknown | 5 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Medicine and Dentistry | 2 | 20% |
Unknown | 6 | 60% |